Development and Method Validation for Testosterone and Epitestosterone in Human Urine Samples by Liquid Chromatography Applications
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چکیده
منابع مشابه
Development and method validation for testosterone and epitestosterone in human urine samples by liquid chromatography applications.
An isocratic high-performance liquid chromatographic method for the determination of testosterone (T) and epitestosterone (ET) in human urine using liquid-liquid or solid-phase extraction (SPE) is developed and validated. The optimum separation is achieved using a Hypersil C(18) column, water-acetonitrile (57:43, v/v) as the mobile phase and UV-absorbance detection at 245 nm. The recoveries obt...
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hydrochlorothiazide (hct) is a diuretic agent which is shown to be effective in the treatment of hypertension. literature reports have demonstrated that urinary excretion data may be used to assess the bioavailability of various formulations containing this thiazide. also hct consumption by the athletes is one of the drugs which should be regulated by world anti-doping agency (wada), because of...
Method development validation for corticoids in animal feed samples by liquid chromatography using a monolithic column.
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The present study describes a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous determination of S-RRR and R-SSS nebivolol (nebivolol enantiomers) in human plasma using solid phase extraction technique. Method of both S-RRR and R-SSS nebivolol (nebivolol enantiomers) has been developed and validated using racemic nebivolol D4 as an internal standard. Analytes...
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ژورنال
عنوان ژورنال: Journal of Chromatographic Science
سال: 2003
ISSN: 0021-9665,1945-239X
DOI: 10.1093/chromsci/41.5.261